Vitamin D levels in cells, not blood, is important (IVF follicular fluid in this case)

Created August 2021

Low vitamin D levels in follicular fluid, but not in serum, are associated with adverse outcomes in assisted reproduction

Arch Gynecol Obstet. 2021 Aug 8. doi: 10.1007/s00404-021-06174-9

Kahindo P Muyayalo # 1 2, Su Song # 3, Hui Zhai # 4, Hong Liu 1, Dong-Hui Huang 1, Hui Zhou 1, Yang-Jiao Chen 3, Ai-Hua Liao 5

Purpose: To assess the relationship between serum/follicular fluid (FF) vitamin D (VD) status and assisted reproductive technology (ART) treatment outcomes among infertile patients.

Methods: A prospective cohort study, including 132 infertile patients scheduled for their first ART treatment cycle, was carried out in a Reproductive Medical Center. Serum and FF samples were collected to assess 25-hydroxy VD [25(OH)D] levels. Low VD level was defined as 25(OH)D concentration of less than 30 ng/mL.

Results: Most infertile patients had low VD levels in serum (88%) and FF (90%). We observed a moderately positive correlation between VD levels in serum and FF (r = 0.34, p < 0.0001). Compared to the group of patients with low VD levels in the FF, those with sufficient VD levels had a significantly higher number of retrieved oocytes (p = 0.03), normal fertilization (p = 0.01), and high-quality embryos (p = 0.001). Moreover, patients with sufficient VD levels in the FF also had significantly higher implantation rates than those with low VD levels (76.92% vs. 46.58%, respectively, p = 0.01) and clinical pregnancy rates (92.31% vs. 61.54%, respectively, p = 0.04).

Conclusion: These data collectively revealed that low VD levels in serum and FF were common among infertile patients.

VD levels in FF, but not in serum, were associated with:

  • embryo quality,

  • normal fertilization,

  • implantation rates, and

  • clinical pregnancy rates.

Further studies are mandatory to determine the molecular mechanism and VD's potential therapeutic benefits in infertile patients.

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Related - In vitro fertilization is more successful when vitamin D in ovaries, not just blood, is increased – April 2022

VitaminDWiki comments

In 2018 the founder of VitaminDWiki asked if it were technically possible to measure vitamin D levels in cells

In 2019 the question was asked again - and the answer then was yes, but it cost about 3X of the serum test

In 2021 most doctors and researchers still only rarely consider the cell levels of vitamin D

5 genes can restrict Vitamin D in the blood from actually getting to the cells

image

The Vitamin D Receptor is perhaps the most important gene

    (the most likely to be deactivated AND the most easily reactivated)

Many diseases have "learned" how to protect themselves by deactivating the Vitamin D Receptor

Many Cancers for example

80-100 ng often gets Vitamin D to the cells even when there are gene restrictions

A normally good level of Vitamin D (e.g. 30 ng) can be restricted by genes however

Vitamin D Receptor category has the following

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VitaminDWiki - Fertility and Sperm category contains

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VitaminDWiki - Vitamin D greatly improves Fertility__

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Tags: CYP27B1 Fertility and sperm Genetics Vit D Binding Protein Vit D Receptor