Vitamin D test using electrospun nanofibers (looks promising)

Created July 2018

Electrochemical immunosensor based on magnetite nanoparticles incorporated electrospun polyacrylonitrile nanofibers for Vitamin-D3 detection

Materials Science and Eng: C, Vol 93, 1 Dec 2018, Pages 145-156, https://doi.org/10.1016/j.msec.2018.07.036

Perhaps this could provide instant vitamin D testing by a machine * Low-cost Vitamin D testers (two yes-no tests for 11 dollars) - 2024 * Who needs a vitamin D test -Those with symptoms or those in high risk groups - March 2018 * Low cost vitamin D Blood Tests 1. Tests for Vitamin D contains the following overview/opinion {include}

PDF is available free at Sci-Hub  10.1016/j.msec.2018.07.036

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In the present study, magnetite nanoparticles (Fe3O4 NPs) incorporated polyacrylonitrile nanofibers (PANnFs) having diameter of 350–500 nm, were electrospun and directly collected onto indium tin oxide (ITO) coated glass substrate. X-ray diffraction pattern of Fe3O4-PANnFs confirmed the existence of Fe3O4 NPs within the PANnFs. Nafion was used to make Fe3O4-PANnFs more adhesive to the ITO surface. Partial hydrolyzation of Fe3O4-PANnFs/ITO electrode was done using NaOH solution for the partial conversion of nitrile group (C≡N) into carboxyl and amine groups that was confirmed by Fourier transform infrared spectroscopy study. The hydrolyzed Fe3O4-PANnFs/ITO electrode was used as an immobilization matrix for monoclonal antibody specific to Vitamin-D3 (Anti-VD) via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-Hydroxysuccinimide chemistry. Bovine serum albumin (BSA) was used as a blocking agent to block the non-specific sites onto Anti-VD/Fe3O4-PANnFs/ITO electrode surface. Fabricated BSA/Anti-VD/Fe3O4-PANnFs/ITO immunoelectrode showed improved biosensing parameters for Vitamin-D3 detection such as sensitivity of 0.90 μA ng−1 mL cm−2, limit of detection of 0.12 ng mL−1and detection range of 10–100 ng mL−1. The association and dissociation constants were obtained as 74.62 ng mL−1, 4.6 ng mL−1, respectively.

Tags: Tests