Serum 25(OH)D concentration, common variants of the VDR gene and lung cancer occurrence.
Int J Cancer. 2017 Jul 15;141(2):336-341. doi: 10.1002/ijc.30740. Epub 2017 Apr 24.
Gromowski T1, Gapska P1, Scott RJ2, Kąklewski K1, Marciniak W3, Durda K1, Lener M1, Górski B1, Cybulski C1, Sukiennicki G1, Kaczmarek K1, Jaworska-Bieniek K1, Paszkowska-Szczur K1, Waloszczyk P4, Lubiński J1,3, Dębniak T1.
- 1 Departmentof Genetics and Pathology, International Hereditary Cancer Center, Pomeranian Medical University, Szczecin, Poland.
- 2 Discipline of Medical Genetics, Faculty of Health, University of Newcastle and Hunter Medical Research Institute, Newcastle, NSW, Australia.
- 3 Read-Gene S.A., Grzepnica, Dobra Szczecińska, Poland.
- 4 Independent Laboratory of Pathology, Zdunomed, Szczecin, Poland.
The first aim of our study was to examine the association between common variants in VDR [rs2228570 (FokI), rs1544410 (BsmI), rs7975232 (ApaI), rs731236 (TaqI) and rs11568820 (Cdx2)] and lung cancer risk in the Polish population. Genotyping and statistical analysis which included Chi-square test with Yates correction and haplotype frequency analysis were performed on a series of 840 consecutively collected lung cancer patients and 920 healthy controls. The second aim was to evaluate the link between serum 25(OH)D concentration and the number of lung cancers in a subgroup of 200 patients. A separate control group that consisted of 400 matched (by age, sex, smoking habits and the season of blood collection) healthy individuals was used to avoid posterior adjustment on the matched variables. Statistical analysis with the use of Chi-square test with Yates was performed.
We found no statistically significant difference in the distribution of the allels of studied VDR variants among cases and controls.
A statistically significant over-representation of VDR haplotypes:
- rs731236_A + rs1544410_T [odds ratio (OR) = 2.43, 95% confidence interval (CI) = 1.11-5.32, p < 0.001],
- rs731236_G + rs1544410_T (OR = 1.54, 95% CI = 1.31-1.81, p < 0.001) and
- rs731236_G + rs1544410_C (OR = 0.04, 95% CI = 0.03-0.07, p < 0.001)
was detected. We found a tendency toward an increased number of lung cancers among individuals with low serum levels of 25(OH)D. To answer the question, whether VDR can be regarded as lung cancer susceptibility gene and low 25(OH)D serum levels is associated with lung cancer occurrences, additional, multicenter study needs to be performed.
PMID: 28411367 DOI: 10.1002/ijc.30740